Development of a novel selective medium for culture of Gram-negative bacteria

Abstract Objective Although many bacterial culture media are available commercially, there is a continuous effort to develop better selective for bacteria, which cannot be grown on existing media. While exploring antibacterial properties of clove, we observed that it has the potential selectively inhibit growth certain types bacteria. This led us do experiments, resulted in developing new allowed only Gram-negative while inhibiting Gram-positive Results Mueller Hinton Agar (MHA) was used as base and modified MHA-C15 (MHA containing 15% volume/volume water extract clove). pathogens Escherichia coli , Klebsiella pneumoniae Salmonella typhimurium Pseudomonas aeruginosa grew MHA-C15. However, none major such Staphylococcus aureus Streptococcus pyogenes mutans, Bacillus spp. Enterococcus it. Taken together, these findings show newly developed